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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
26/01/2024 |
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Actualizado : |
26/01/2024 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
PICCOLI, R.J.; GRUCHOUSKEI, L.; BENINCA, A.L.V.; MAURENTE BERÓN, M.; CHENG, A.C.; DE ANDRADE, J.A.; FACCIN, M.; GRZEGOZEVSKI, A.P.; DA SILVA, G.C.R.; FRANÇA, J.C.; FERNANDES, N.L.M.; DE CARVALHO, A.L.; VIOTT, A.M. |
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Afiliación : |
RONALDO JOSÉ PICCOLI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; LEONARDO GRUCHOUSKEI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDRÉ LUIS VRIESMAN BENINCA, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MARINA MAURENTE BERÓN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ARTHUR COLOMBARI CHENG, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JOICE APARECIDA DE ANDRADE, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MAYANE FACCIN, Veterinary Pathobiology Department, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States; ALINE PATRÍCIA GRZEGOZEVSKI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; GEÓRGIA CAROLINA ROHDEN DA SILVA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JAQUELINE COELHO FRANÇA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; NELSON LUIS MELLO FERNANDES, Immunological Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDERSON LUIZ DE CARVALHO, Veterinary Hospital, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ALINE DE MARCO VIOTT, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil. |
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Título : |
Detection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species. |
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Complemento del título : |
Original full article. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Brazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS. |
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ISSN : |
1983-0246 |
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DOI : |
10.0.94.6/bjvp.1983-0246.v16i3p159-166 |
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Idioma : |
Inglés |
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Notas : |
Article history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br -- |
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Contenido : |
ABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved. MenosABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additio... Presentar Todo |
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Palabras claves : |
Diagnosis; Immunohistochemistry; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Psittacosis; Zoonosis. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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URL : |
https://bjvp.org.br/wp-content/uploads/2023/10/BJVP_v16n3_159-166.pdf
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Marc : |
LEADER 03124naa a2200361 a 4500
001 1064440
005 2024-01-26
008 2023 bl uuuu u00u1 u #d
022 $a1983-0246
024 7 $a10.0.94.6/bjvp.1983-0246.v16i3p159-166$2DOI
100 1 $aPICCOLI, R.J.
245 $aDetection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species.$h[electronic resource]
260 $c2023
500 $aArticle history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br --
520 $aABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved.
653 $aDiagnosis
653 $aImmunohistochemistry
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aPsittacosis
653 $aZoonosis
700 1 $aGRUCHOUSKEI, L.
700 1 $aBENINCA, A.L.V.
700 1 $aMAURENTE BERÓN, M.
700 1 $aCHENG, A.C.
700 1 $aDE ANDRADE, J.A.
700 1 $aFACCIN, M.
700 1 $aGRZEGOZEVSKI, A.P.
700 1 $aDA SILVA, G.C.R.
700 1 $aFRANÇA, J.C.
700 1 $aFERNANDES, N.L.M.
700 1 $aDE CARVALHO, A.L.
700 1 $aVIOTT, A.M.
773 $tBrazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
04/01/2021 |
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Actualizado : |
24/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
-- - -- |
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Autor : |
SOUSA, D. E. RIBEIRO DE; BARBOSA, E. DE F. GAUDÊNCIO; WILSON, T. MEZIARA; MACHADO, M.; OLIVEIRA, W. JÚNIOR; DUARTE, M. ALMEIDA; SCALON, M. CORRÊA; CÂMARA, A. C. LOPES; HOPPE, E. G. LUX; PALUDO, G. R.; MELO, C. BARROS DE; CASTRO, M. BOTELHO DE |
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Afiliación : |
DAVI EMANUEL RIBEIRO DEDSOUSA, Veterinary Pathology Laboratory, Campus Darcy Ribeiro, University of Brasília. Brasília, Brasil.; EDSON DE FIGUEIREDO GAUDÊNCIO BARBOSA, Centro Universitário CESMAC, Faculdade Cesmac do Sertão, CESMAC UNIDADE MARECHAL DEODORO, BRASIL.; TAIS MEZIARA WILSON, eterinary Pathology Laboratory, Campus Darcy Ribeiro, University of Brasília. Brasília, Brazil.; MIZAEL MACHADO DA COSTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; WILSON JÚNIOR OLIVEIRA, Department of Pathology, Animal Reproduction, and One Health Laboratory of Parasitic Diseases – LabEPar, Paulista State University, Brazil; MATHEUS ALMEIDA DUARTE, Veterinary Clinical Pathology Laboratory, Campus Darcy Ribeiro, University of Brasília. Brasília, Brazil; MARCELA CORRÊA SCALON, Veterinary Clinical Pathology Laboratory, Campus Darcy Ribeiro, University of Brasília. Brasília, Brazil.; ANTÔNIO CARLOS LOPES CÂMARA, Large Animal Veterinary Teaching Hospital, University of Brasília (UnB), Brasília, Brazil.; ESTEVAM GUILHERME LUX HOPPE, Department of Pathology, Animal Reproduction, and One Health Laboratory of Parasitic Diseases, LabEPar, Paulista State University, Brazil.; GIANE REGINA PALUDO, Veterinary Clinical Pathology Laboratory, Campus Darcy Ribeiro, University of Brasília, Brazil.; CRISTIANO BARROS DE MELO, Postgraduate Program in Animal Sciences, College of Agronomy and Veterinary Medicine, University of Brasília, Brasília, Brazil.; MÁRCIO BOTELHO DE CASTRO, Veterinary Pathology Laboratory, Campus Darcy Ribeiro, University of Brasília. Brasília, Brazil. |
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Título : |
Eurytrema coelomaticum natural infection in small ruminants: A neglected condition. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Parasitology: 15 Jan. 2021. DOI: https://doi.org/10.1017/S0031182020002358 |
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Páginas : |
8 p. |
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DOI : |
10.1017/S0031182020002358 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 26 September 2020 // Revised: 3 December 2020// Accepted: 8 December 2020// Published: 12 January 2021.
E-mail address: mbcastro@unb.br |
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Contenido : |
Abstract: Pancreatic eurytrematosis (PE) is an under diagnosed and neglected parasitosis in goats and sheep in the Americas. Clinical and pathological features of PE are not well defined in small ruminants worldwide. Natural cases of PE in small ruminants were detected in the Federal District, Brazil. A survey of necropsy records, including epidemiological and clinicopathological data, in goats and sheep was conducted. Most cases of PE occurred during the rainy season in adult females, with an incidence of 12.9% in goats and 0.8% in sheep. Clinical signs varied from asymptomatic infections to anorexia, lethargy, weakness, marked weight loss and death in some goats. Overall, most cases of PE in goats and sheep were incidental necropsy findings with minor pancreatic lesions. Three goats, however, showed severe chronic pancreatitis, dilation of major pancreatic ducts with numerous trematodes present and marked abdominal fat necrosis. Morphological and molecular characterization of flukes
detected Eurytrema coelomaticum. Our findings shed light on the prevalence of E. coelomaticum infections in small ruminants in the region and highlight the possibility of severe and lethal cases in goats. PE must be further investigated in small ruminant populations in relevant livestock production regions of the Americas. |
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Palabras claves : |
ENFERMEDADES DE LOS ANIMALES; ENFERMEDADES DE LOS RUMIANTES; EURYTREMA COELOMATICUM; EURYTREMATOSIS; FAT NECROSIS; GOAT; PANCREAS; SHEEP; TREMATODE. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 02657naa a2200397 a 4500
001 1061644
005 2021-06-24
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1017/S0031182020002358$2DOI
100 1 $aSOUSA, D. E. RIBEIRO DE
245 $aEurytrema coelomaticum natural infection in small ruminants$bA neglected condition.$h[electronic resource]
260 $c2021
300 $a8 p.
500 $aArticle history: Received: 26 September 2020 // Revised: 3 December 2020// Accepted: 8 December 2020// Published: 12 January 2021. E-mail address: mbcastro@unb.br
520 $aAbstract: Pancreatic eurytrematosis (PE) is an under diagnosed and neglected parasitosis in goats and sheep in the Americas. Clinical and pathological features of PE are not well defined in small ruminants worldwide. Natural cases of PE in small ruminants were detected in the Federal District, Brazil. A survey of necropsy records, including epidemiological and clinicopathological data, in goats and sheep was conducted. Most cases of PE occurred during the rainy season in adult females, with an incidence of 12.9% in goats and 0.8% in sheep. Clinical signs varied from asymptomatic infections to anorexia, lethargy, weakness, marked weight loss and death in some goats. Overall, most cases of PE in goats and sheep were incidental necropsy findings with minor pancreatic lesions. Three goats, however, showed severe chronic pancreatitis, dilation of major pancreatic ducts with numerous trematodes present and marked abdominal fat necrosis. Morphological and molecular characterization of flukes detected Eurytrema coelomaticum. Our findings shed light on the prevalence of E. coelomaticum infections in small ruminants in the region and highlight the possibility of severe and lethal cases in goats. PE must be further investigated in small ruminant populations in relevant livestock production regions of the Americas.
653 $aENFERMEDADES DE LOS ANIMALES
653 $aENFERMEDADES DE LOS RUMIANTES
653 $aEURYTREMA COELOMATICUM
653 $aEURYTREMATOSIS
653 $aFAT NECROSIS
653 $aGOAT
653 $aPANCREAS
653 $aSHEEP
653 $aTREMATODE
700 1 $aBARBOSA, E. DE F. GAUDÊNCIO
700 1 $aWILSON, T. MEZIARA
700 1 $aMACHADO, M.
700 1 $aOLIVEIRA, W. JÚNIOR
700 1 $aDUARTE, M. ALMEIDA
700 1 $aSCALON, M. CORRÊA
700 1 $aCÂMARA, A. C. LOPES
700 1 $aHOPPE, E. G. LUX
700 1 $aPALUDO, G. R.
700 1 $aMELO, C. BARROS DE
700 1 $aCASTRO, M. BOTELHO DE
773 $tParasitology: 15 Jan. 2021. DOI: https://doi.org/10.1017/S0031182020002358
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